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Title:Limiting Dilution Analysis Of Cells Of The Immune System
Format Type:Ebook
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Publisher:eBooks
ISBN:0198501285
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Limiting Dilution Analysis Of Cells Of The Immune System by Ivan Lefkovits

PDF, EPUB, MOBI, TXT, DOC Limiting Dilution Analysis Of Cells Of The Immune System This new edition is an extensive treatise on the rationale of limiting dilution analysis LDA LDA has evolved from its previous role as a basic research laboratory tool in virology and immunology to one pertaining to many different research disciplines and to clinical applications New methodologies such as PCR confocal microscopy in situ hybridisation have emerged with the result that single cell assays can now be performed on a more defined basis and the quantitation of cells and their precursors is now more accessible to the limiting dilution approach This book includes both detailed mathematical derivations and methodological descriptions so that the uninitiated scientist can set up and evaluate limiting dilution experiments without further instruction It also describes particular examples of how LDA is being used to determine clinical practice The second edition of this highly successful book which has been on the shelf of every modern immunology laboratory for the last two decades retains the same overall structure of the first edition with one important addition an extensive LDA software package The LDA program and the simulation software will be linked to a web site to ensure updates at regular intervals The first edition was written primarily for immunologists but this volume has been expanded so that it will be useful to a broader audience in microbiology pathology virology and cellular biology Furthermore this volume is intended to be used as additional reading for students of biology and medicine whose knowledge of the applications of Poisson distribution need refreshing

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Portrait of the Immune System, A: Scientific Publications of N K Jerne, Immunological Methods, Volume 2, Limiting Dilution Analysis of Cells in the Immune System, Immunological Methods, Volume 1, The Immune System: Festschrift in Honor of Niels Kaj Jerne, on the Occasion of His 70th Birthday, Immunological Methods, Volume 4, Immunological Methods, Volume 3, Immunology Methods Manual, Four-Volume Set: The Comprehensive Sourcebook of Techniques, Portrait of the Immune System, A: Scientific Publications of N K Jerne, Immunological Methods
The Immunology Methods Manual is a four volume set comprising a comprehensive sourcebook of immunological methods and techniques Unique in its project oriented rather than protocol oriented approach the emphasis is placed on describing the environment in which each method was developed and is used The manual contains both advanced methods and descriptions and basic protocols It is essential not only for those who study the immune system of man or mouse but also those applying methods to other species This new compilation of methods answers the needs of immunologists molecular developmental and cell biologists students and advanced researchers and workers in clinical and diagnostic laboratories Protocols are provided to guide day to day laboratory work as well as the development of long term strategies Vital contact information also encourages useful dialogue and interaction between authors and method users br More than section editors and authors have contributed to compile this four volume practical guide to methods that work br Key Features br Provides essential hands on methods br Presents protocols with background information br Ideally suited for use in a laboratory br Covers all molecular and cellular techniques br Key Features of the CD ROM br Compatible with Windows Mac and UNIX platforms br Features a full range of advanced search capabilities br Includes a personalized note creation feature enabling the user to add bookmarks and post its br Offers full cut copy and paste capabilities allowing printing exporting and editing br Provides hyperlinks between solutions reagents and their recipes figures tables and their text citations and reference citations to their bibliographic listings br Contains a Pathway feature which allows recording of content during a particular session or loading a pre recorded tutorial br Includes the animated film The Immune System by Andre Traunecker and Stefan Meyer, Using the published work of Nobel Laureate Niels Kaj Jerne this book shows how he developd his ideas The book is a compilation of his published work but in fact it is much more than that Whether the reader wants to read the book systematically or only browse it opens a fascinating world of hypotheses theories facts and vistas His selection theory his view of how immunological diversity is created and his concept of lymphocytes interacting as a network reveals Jerne s revolutionary spirit The book ought to be a rich source of inspiration for everyone interested in science and how science is made, Immunological Methods a compendium of basic research techniques being used in one of the largest immunology research institutes the Basel Institute for Immunology with particular emphasis given to new methodology The procedures have been described by individuals judged to be highly expert in their specialties In many instances the methods developed or adapted to unique uses by the contributors have not previously been described in detail br The book contains chapters covering techniques for detection isolation and purification of antibodies including dansylation two dimensional chromatography isoelectric focusing polyacrylamide gel electrophoresis and isotachophoresis measurement of equilibrium constants equilibrium dialysis filtration and sedimentation and isotope and fluorescent labeling and detection of cell surface components Techniques such as isotope laboratory maintenance chemical modification of proteins haptens and solid supports and haptenation of viable biological carriers production of antisera against allotypes and histocompatibility antigens and production of antibody with clonai dominance histocompatibility and MLR testing and cell separation by haptenated gels and by velocity sedimentation of rosette forming cells are also discussed Other chapters cover detection of antibody secreting and alloantigen binding cells immune responses in vitro and their analysis by limiting dilution production of T cell factors hybridoma production by cell fusion maintenance of cell lines and cloning in semisolid media and the mathematical analysis of immunological data, No description available